pbluescript sk (2) phagemid vectors (Agilent technologies)

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Agilent technologies pbluescript sk (2) phagemid vectors
Pbluescript Sk (2) Phagemid Vectors, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+sk(2)+phagemid+vector/pm09305910-71-17-22
Average 90 stars, based on 1 article reviews

pbluescript sk (2) phagemid vectors - by Bioz Stars, 2026-09

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Plasmid Preparation:

Article Title: New beta-hydroxyaspartate derivatives are competitive blockers for the bovine glutamate/aspartate transporter.
Article Snippet: Expression of EAAT1 in Oocytes—Capped RNA was transcribed from linearized pBluescript II SK(2) phagemid vector (Stratagene) containing the coding region for EAAT1 (9, 12) using T3 polymerase (Stratagene).

Article Title: Cloning and Functional Expression of a Brain Peptide/Histidine Transporter
Article Snippet: Insert cDNAs in positive phages were recirculated to the subclone in the pBluescript SK(2) phagemid vector after superinfection with R408 helper phages as recommended by the manufacturer (Stratagene).

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Clone Assay:

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Variant Assay:

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Over Expression:

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Acetylene Reduction Assay:

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Expressing:

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: BL21(DE3) F dcm ompT hsdS(rB mB ) gal met (DE3) Novagen ER2058 F ara-14 leuB6 fhuA2 (argF-lac)U169 lacY1lon::mini-Tn10(Tetr) glnV44 galK2 rpsL20(Strr)xyl-5 mtl-5 (malB) zjc::Tn5(Kanr) (mcrC-mrr)HB101 New England Biolabs ET12567/pUZ8002 dam-13::Tn9 dcm-6 hsdM; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac F proAB lacIqZ M15 Tn10(Tetr) Stratagene pBluescript II SK( ) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK( ) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38

Article Title: Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
Article Snippet: Staphylococcus aureus (ATCC 29213) was grown on Oxoid nutrient agar (ONA) with or without 12 mM calcium nitrate. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain/plasmid Genotype/description Reference or source E. coli BL21(DE3) F − dcm ompT hsdS (r B − m B − ) gal met λ (DE3) Novagen ER2058 F − ara - 14 leu B6 fhu A2 Δ( argF - lac ) U169 lacY1 lon ::mini-Tn 10 (Tet r ) glnV44 galK2 rpsL20 (Str r ) xyl-5 mtl-5 Δ( malB ) zjc ::Tn 5 (Kan r ) Δ( mcrC-mrr ) HB101 New England Biolabs ET12567/pUZ8002 dam-13 ::Tn 9 dcm -6 hsdM ; containing the nontransmissible oriT mobilizing plasmid pUZ8002 16 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z Δ M15 Tn 10 (Tet r )] Stratagene pBluescript II SK(+) Phagemid cloning vector Stratagene pPCR-Script Amp Phagemid cloning vector; variant ofpBluescript II SK(+) containing a SrfI site for insertion of blunt-end amplicons Stratagene pMAL-c2X Protein overexpression vector resulting in an N-terminal MBP fusion; Tac promoter New England Biolabs pMALA2 absA2 in pMAL-c2X 38 S. coelicolor J1501 hisA1 uraA1 strA1 pgl SCP1 SCP2 26 C542 J1501 hisA1 uraA1 strA1 pgl absA1-542 SCP1 SCP2 3 pIJ6902 Integrative P tipA expression vector ori pUC18 oriT RK2 int φC31 attP tsr aac ( 3 ) IV 23 p act II-ORF4 act II-ORF4 in pIJ6902 This work p cdaR cdaR in pIJ6902 This work p redZ redZ in pIJ6902 This work Open in a separate window Bacterial strains and plasmids used in this study

Review